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human normal lung bronchial epithelial cell line  (ATCC)


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    ATCC human normal lung bronchial epithelial cell line
    Human Normal Lung Bronchial Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human normal lung bronchial epithelial cell line/product/ATCC
    Average 99 stars, based on 4170 article reviews
    human normal lung bronchial epithelial cell line - by Bioz Stars, 2026-02
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    Heat map of differentially expressed genes based on PCR Array analysis. NHBE cells were transfected with vector, hC/EBPβ, control siRNA or hC/EBPβ siRNA for 24h. Cells were then mock infected or infected with PR8 (1.0 MOI) for 6 h. Gene expression were analysed by real-time RT-PCR using PCR array kit as per manufacturer protocol. Data shown are average from two independent experiments. The scale shows the level of gene expression where red corresponds to upregulation (log2 fold).

    Journal: Antiviral research

    Article Title: Influenza virus NS1- C/EBPβ gene regulatory complex inhibits RIG-I transcription

    doi: 10.1016/j.antiviral.2020.104747

    Figure Lengend Snippet: Heat map of differentially expressed genes based on PCR Array analysis. NHBE cells were transfected with vector, hC/EBPβ, control siRNA or hC/EBPβ siRNA for 24h. Cells were then mock infected or infected with PR8 (1.0 MOI) for 6 h. Gene expression were analysed by real-time RT-PCR using PCR array kit as per manufacturer protocol. Data shown are average from two independent experiments. The scale shows the level of gene expression where red corresponds to upregulation (log2 fold).

    Article Snippet: Human lung epithelial cell line A549, and normal human bronchial epithelial (NHBE) cells (Lonza, Switzerland) were maintained as described ( Ranjan et al., 2010 ).

    Techniques: Transfection, Plasmid Preparation, Control, Infection, Gene Expression, Quantitative RT-PCR

    PCAT7 promotes metastasis and enhances the epithelial-tomesenchymal transition of NSCLC cells. ( A ) Wound healing assay showed that PACT7 accerlated migration ability (×50); ( B ) Transwell migration assay and Matrigel invasion assay showed that NY-SAR-35 promoted migration/invasion capacity (×50); ( C ) Western blot analysis of E-cadherin, N-cadherin, and Vimentin expression levels. The columns show the mean for 3 separate experiments; bars, sd. * P<0.05. ** P<0.01

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Non-Coding RNA Prostate Cancer-Associated Transcript 7 (PCAT7) Induces Poor Prognosis and Promotes Tumorigenesis by Inhibiting mir-134-5p in Non-Small-Cell Lung (NSCLC)

    doi: 10.12659/MSM.907904

    Figure Lengend Snippet: PCAT7 promotes metastasis and enhances the epithelial-tomesenchymal transition of NSCLC cells. ( A ) Wound healing assay showed that PACT7 accerlated migration ability (×50); ( B ) Transwell migration assay and Matrigel invasion assay showed that NY-SAR-35 promoted migration/invasion capacity (×50); ( C ) Western blot analysis of E-cadherin, N-cadherin, and Vimentin expression levels. The columns show the mean for 3 separate experiments; bars, sd. * P<0.05. ** P<0.01

    Article Snippet: We purchased 6 NSCLC cell lines (A549, H292, H460, H1703, H358, and H1975) and a normal human lung bronchial epithelial cell line (16-HBE) from the American Type Culture Collection (ATCC, USA) and cultured them in RPMI-1640 Medium (Gibco BRL, Gaithersburg, MD) with 10% fetal bovine serum, ampicillin, and streptomycin in a humidified atmosphere of 95% air and 5% CO 2 , with the temperature at 37°C.

    Techniques: Wound Healing Assay, Migration, Transwell Migration Assay, Invasion Assay, Western Blot, Expressing